Hanna Instruments Marine Instruments Turbidity and Free/Total Chlorine Meter User Manual

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CALIBRATION PROCEDURE
TURBIDIMETER CALIBRATION
HI 93414 has a powerful calibration function that compensates for lamp aging or changing. The
calibration can be done using the suplied calibration solutions or user prepared standards.
HI 93414 turbidimeter is supplied with 4 AMCO standards –<0.1 NTU, 15 NTU, 100 NTU and 750
NTU. The Hanna standards are specially designed for this instrument. Turbidity standards have a shelf
life and should not be used after the expiration date.
Alternatively, formazin standards can be used. It is recommended that the turbidity value of the
prepared calibration solutions to be close to the default calibration points.
The first calibration point should be near 0 NTU, the second point can be chosen between 10 and
20 NTU, the third point between 50 and 150 NTU and the fourth point between 600 and 900 NTU.
FORMAZIN PREPARATION
In order to prepare formazin 4000 NTU stock solution, follow the next procedure:
Solution I : Dissolve 1.000 grams of hydrazine sulfate, (NH
2
)
2
H
2
SO
4
, in distilled, deionized
water and dilute to 100 mL in a volumetric flask.
Warning: Handle hydrazine sulfate with care because it is a carcinogen reagent. Avoid inhalation,
ingestion, or skin contact.
Formazin solution can also contain some hydrazine traces.
Solution II : Dissolve 10.000 grams of hexamethylenetetramine, (CH2)
6
N
4
, in distilled, deionized
water and dilute to 100 mL in a volumetric flask.
Stock solution: Mix 10 mL Solution I and 10 mL Solution II in a flask. Let the stock solution stays
48 hours at 25±3°C (77±5°F). This will result in a 4000 NTU formazin suspension. It is very
important for the formation of the formazin polymer to maintain the same temperature.
The stock solution (4000 NTU) can be stored up to one year in proper conditions. Store formazin in
amber glass bottle or any UV-light blocking bottle.
To obtain a high quality formazin always use pure reagents and high-purity water.
To prepare the calibration standards, dilute the stock solution with the same high-purity water you
used for the preparation of the stock solution.
The diluted formazin solutions are not stable. They should be used immediately after preparation
and discard immediately after use.
CALIBRATION
For best results, the measurement techniques must be followed during calibration. If formazin
standards are used, mix the cuvets gently for about 1 minute and then allow the standard to settle for
1 more minute before calibration.
Calibration can be performed in two, three or four points. It is possible to interupt calibration
procedure at any time by pressing CAL/CHECK or ON/OFF.
Note: Calibration of the turbidity range will not affect the free or total chlorine measurements.

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