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20
HEATING THE SAMPLE
Use of heat to remove air bubbles, although very effective in some cases, should be handled with care
because it can alter the turbidity of the sample. When heating a sample, the volatile components
from the sample can vaporize, the suspended components can dissolve or the sample characteristics
can change.
Therefore, the heating procedure should be used with extreme care.
The best way is to use a warm water bath and immerse the cuvet with sample into the bath. Heat the
sample only until the visible bubbles are removed.
Note: Always cool the heated sample to the original sample temperature before measurement.
The heating procedure can be used in combination with vacuum or ultrasonic waves application for
a more effective air bubble removal.
REAGENT ADDING (COLORIMETRY only)
Because the reagent quantity is set up to react with 10mL of
sample is very important to fill the cuvet correctly. The liquid in
the cuvet forms a convexity on the top; the bottom of this convexity
must be at the same level with the 10 mL mark.
To open the powder reagent pack:
a) use scissors to open the powder packet
b) push the edges of the packet to form a spout
c) pour the content of the packet
Do not let the reacted sample to stand too long after reagent is added or accuracy will be lost.
All the reaction times reported in this manual are reffered to 20ºC (68ºF). As a general rule of
thumb, they should be doubled at 10ºC (50ºF) and halved at 30ºC (86ºF).
Insert the cuvet with the mark aligned with the mark on the instrument top.
It is possible to take multiple readings in a row but it is recommended to take a new zero reading
for each sample and to use the same cuvet for zeroing and measurement.
After the reading it is important to discard immediately the sample, otherwise the glass might
become permanently stained.
Note: In order to maximize accuracy, prior to a measurement follow the validation procedure, to
be sure that the instrument is properly calibrated. If necessary, calibrate the instrument.